Journal: Science Advances

Article Title: Moderate embryonic delay of paternal mitochondrial elimination impairs mating and cognition and alters behaviors of adult animals

doi: 10.1126/sciadv.adp8351

Figure Lengend Snippet: ( A ) A schematic diagram showing the assay for male mating efficiency using fog-2(q71) females. Feminized fog-2(q71) animals produce only eggs and no sperm and could not conceive if no male is present. In each mating plate, five fog-2(q71) females were placed with one day-2 adult male and allowed to mate for 1.5 hours. The number of conceived fog-2(q71) females with eggs in the uterus was then scored using a Nomarski microscope. The mating efficiency (%) of the tested male is determined by the number of conceived fog-2 females/5 × 100%. ( B ) Mating efficiencies of F1 males from the indicated crosses. The number of F1 males tested in each cross is shown inside or above the bar. ( C ) Representative movement tracks of day-1 adult hermaphrodite animals or day-1 adult hermaphrodite cross-progeny from the indicated crosses. In each experiment, 10 adult animals were allowed to freely move in a testing NGM plate that had been seeded with a thin layer of OP50 bacteria and tracked by the Wormlab Acquisition System. Each recording time was 1 minute. ( D and E ) Peristaltic speed (D) and peristaltic track length (E) of hermaphrodite animals or hermaphrodite cross-progeny from the indicated crosses are shown. Each recording time was 1 min. The number of animals analyzed in each experiment is shown inside the bar. In (B), (D), and (E), data are mean ± SEM; * P < 0.05, ** P < 0.01, and ** P < 0.001 (unpaired Student t test); ns, not significant. cps-6(tm3222) was used where cps-6 is indicated, while cps-6(sm493) was used where HA cps-6 is indicated.

Article Snippet: The peristaltic speed and track length of C. elegans movement were measured by the Wormlab Acquisition System (MBF Bioscience), equipped with a high-resolution camera with a zoom lens and corresponding software.

Techniques: Microscopy, Bacteria